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       CHO ¼¼Æ÷¸¦ ÀÌ¿ëÇÑ ½Ã½ºÅÛ »ý¹°ÇÐ ¹× ÇÕ¼º »ý¹°ÇÐ ¿¬±¸ µ¿Çâ(KAIST ±è¿¬Á¤, À̱չÎ)
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       À̱չΠ       2013.09.24 11:13        10531
´Ù¿î·Îµå : CHO_¼¼Æ÷¸¦_ÀÌ¿ëÇÑ_½Ã½ºÅÛ_»ý¹°ÇÐ_¹×_ÇÕ¼º_»ý¹°ÇÐ_¿¬±¸µ¿Çâ(KAIST ±è¿¬Á¤, À̱չÎ).pdf(162 Kb)
 

CHO ¼¼Æ÷¸¦ ÀÌ¿ëÇÑ ½Ã½ºÅÛ »ý¹°ÇÐ ¹× ÇÕ¼º »ý¹°ÇÐ ¿¬±¸ µ¿Çâ

 

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½Ã½ºÅÛ »ý¹°ÇÐÀ» ¹ÙÅÁÀ¸·Î ÇÏ¿© CHO ¼÷ÁÖ ¼¼Æ÷ÁÖÀÇ »ý»ê¼º, ¼¼Æ÷ »ýÁ¸À², »ý»êÇ°ÀÇ Ç°ÁúÀÇ °­È­¸¦ À§ÇÑ ´ë»ó À¯ÀüÀÚ È帱º Ž»ö ¹× ÇÕ¼º »ý¹°ÇÐÀû ±â¼úÀ» ÀÌ¿ëÇÏ¿© ¼÷ÁÖ ¼¼Æ÷ÀÇ °­È­¸¦ À§ÇØ ¼±º°µÈ À¯ÀüÀÚ ¸ðµâ Á¦ÀÛ ¹× µµÀÔ Àü·«Àº ÃÖ±Ù ¹àÇôÁø CHO ¼¼Æ÷ À¯ÀüÀÚ ¼­¿­ ¹× ºÐ¼® ±â¼ú°ú À§Ä¡ ƯÀÌÀû À¯Àüü »ðÀÔ ±â¼ú (Zinc Finger Nuclease, ZFN) µîÀ» ÀÌ¿ëÇØ °¡´ÉÇØÁú °ÍÀ¸·Î ¿¹»óÇÑ´Ù. º» ¿¬±¸ÆÀÀº ½Ã½ºÅÛ »ý¹°ÇÐÀ» ±â¹ÝÀ¸·Î ÇÏ¿© ±âÁ¸ ¼¼Æ÷ÁÖ ±¸Ãà ¹æ½ÄÀÇ ÇѰ踦 ±Øº¹ÇÒ ¼ö ÀÖÀ» °ÍÀ¸·Î Àü¸ÁµÈ´Ù. CHO ¼¼Æ÷ engineering ÀÇ ÃÖ±Ù ¼Ò½ÄµéÀ» °¡Àå Àß Á¢ÇÒ ¼ö ÀÖ´Â Cell Culture Engineering XIII ÇÐȸ Âü¼®À» ÅëÇÏ¿© CHO ¼¼Æ÷¸¦ ÀÌ¿ëÇÑ ½Ã½ºÅÛ »ý¹°Çаú ÇÕ¼º »ý¹°ÇÐÀÇ ÃֽŠµ¿Çâ¿¡ ´ëÇؼ­ »ìÆ캸¾Ò´Ù.


 

±×¸²1. CHO genome public database¿Í chromosomal assignment to scaffold

 

½Ã½ºÅÛ »ý¹°Çаú ÇÕ¼º »ý¹°ÇÐÀÇ CHO ¼¼Æ÷·ÎÀÇ Àû¿ë¿¡ °¡Àå ÇʼöÀûÀÎ ¿ä¼ÒÀÎ CHO genome µ¥ÀÌÅÍ º£À̽º°¡ 2011³â Nature biotechnlogy¿¡ °ÔÀçµÇ¾úÀ¸¸ç, Cell Culture Engineering ÇÐȸ¿¡¼­µµ ±× ÀÚ¼¼ÇÑ ³»¿ëÀÌ ¹ßÇ¥µÇ¾ú´Ù. Chinese hamster genome µ¥ÀÌÅͺ£À̽º´Â Chinese hamster ovary ¼¼Æ÷ Ä¿¹Â´ÏƼÀÇ ¿Â¶óÀÎ ¼Ò½ºÀÌ´Ù. FDA ½ÂÀÎÀÌ ¿Ï·áµÈ ½Å±Ô ÀǾàÇ°ÀÇ 1/4 °¡·®ÀÌ CHO ¼¼Æ÷¿¡¼­ »ý»êµÈ °ÍÀ¸·Î ¾Ë·ÁÁ® ÀÖ´Ù. CHO-K1 ¼¼Æ÷ÁÖ Áö³ðÀÇ ¼­¿­ ¹ß°ßÀ» À§ÇØ ±¹Á¦ÀûÀ¸·Î ´Ù¾çÇÑ ³ë·ÂµéÀÌ ÁøÇàµÇ¾ú°í, ´Ù¸¥ ¿¬°üµÈ CHO ¼¼Æ÷ÁÖ ¿Í Chinese hamster  tissueµé¿¡ ´ëÇؼ­µµ ¸¶Âù°¡Áö·Î ÀÌ·ç¾îÁ³´Ù. ÀÌ·¸°Ô ¹ß°ßµÈ CHO ¼¼Æ÷ÁÖÀÇ Áö³ð Á¤º¸µéÀº ¼¼Æ÷ÁÖ engineering °ú ±âÀÛ¿¡ °üÇÑ ´Ù¾çÇÑ ¿¬±¸, ±¸Ã¼ÀûÀ¸·Î´Â °í»ý»ê ȤÀº ¹ÙÀÌ·¯½º ÀúÇ×¼º¿¡ ´ëÇÑ ¼¼Æ÷ÁÖ ¼ºÇâ ¹× Æ¯¼º ºÐ¼®¿¡ °üÇÑ ¿¬±¸ÀÇ È°¼ºÈ­¸¦ µµ¿ï °ÍÀ¸·Î ±â´ëµÈ´Ù. °Ô´Ù°¡, CHO-K1 Áö³ðÀº ÃßÈÄÀÇ genomics, transcriptomic ±×¸®°í proteomic ¿¬±¸ÀÇ ÁÁÀº Âü°íÀÚ·á·Î½á »ç¿ëµÉ °ÍÀÌ´Ù. ¶ÇÇÑ, ±âŸ ´Ù¸¥ ¸ðµ¨ »ý¹°Ã¼ÀÎ Drosophila, mouse, ±×¸®°í rat Ä¿¹Â´ÏƼÀÇ ¹ßÀüµÈ µ¥ÀÌÅÍ º£À̽º¿Í ÇÔ²² CHO Áö³ð µ¥ÀÌÅͺ£À̽º´Â ¸ðµÎ ÇÔ²² CHO ¼¼Æ÷ ±â¼ú °ú genome- scale toolÀÇ ¹ßÀüÀ» ÇâÇÏ¿© ÁøÇàµÉ °ÍÀÌ´Ù. ÃÖ±Ù¿¡, CHO genome ¼­¿­ÀÇ ¹ß°ßÀÇ °á°ú·Î ¾ò¾îÁø µ¥ÀÌÅͺ£À̽ºÀÇ ¼­¿­°ú annotation Á¤º¸´Â ´Ü¹éÁúÀ» ¾ÏȣȭÇÏ´Â 24000°³ÀÇ À¯ÀüÀÚ°¡ Æ÷ÇԵǾî ÀÖ´Ù.


´Ü¹éÁúÀ» ¾ÏȣȭÇÏ´Â °¢°¢ÀÇ ¼¼ºÎ Á¤º¸´Â Áö³ð»óÀÇ À§Ä¡, À¯ÀüÀÚ ¿Í ´Ü¹éÁú ¼­¿­, homologous ´Ü¹éÁú, gene ontology (GO) ¸¦ Æ÷ÇÔÇϸç, Á¤º¸µéÀº public µ¥ÀÌÅͺ£À̽ºÀÎ NCBI GenBank, EMBL-EBK ¿Í UniProt ¿¡¼­ È®ÀÎÇÒ ¼ö ÀÖ´Ù. Searching, browsing, ±×¸®°í ã¾Æº¸´Â Áö³ë¹Í µ¥ÀÌÅ͵éÀÇ ÀÏ·ÃÀº CHO Áö³ë¹Í Á¤º¸¿¡ ´ëÇÑ Á¢±ÙÀ» °­È­Çϴµ¥ »ç¿ëµÉ °ÍÀÌ´Ù. CHO BLAST server´Â ¼­¿­ÀÇ À¯»ç¼ºÀ» ã´Â °ÍÀ» µ½±âÀ§ÇØ ±¸ÃàµÇ¾ú´Ù. ÃßÈÄÀÇ ¼¼°èÀûÀÎ ¿¬±¸ Ãß¼¼·Î´Â µ¥ÀÌÅͺ£À̽ºÀÇ È®ÀåÀ¸·Î, ´Ù¾çÇÑ Áö³ð ½ºÄÉÀÏÀÇ µ¥ÀÌÅÍ º£À̽º¸¦ CHO ¼¼Æ÷·ÎºÎÅÍ ¾ò¾î³»¾î, genomic, transcriptomic, microRNA, proteomic, metabolic, ±×¸®°í microarray µ¥ÀÌÅ͸¦ ÅëÇÏ¿© °¡´ÉÇÒ °ÍÀ¸·Î ¿¹»óÇÑ´Ù. ÀÌ·¯ÇÑ ¿À¹Í µ¥ÀÌÅͺ£À̽ºÀÇ ÅëÇÕÀº genetic engineering Ÿ°ÙÀÇ ¹ß±¼À» ÃËÁøÇÒ °ÍÀ̸ç, ¼­¿­ ƯÀÌÀûÀ¸·Î ÀÛ¿ëÇÏ´Â ±â¼ú¿¡ ´ëÇÑ ¹ßÀüÀ» Çâ»ó½Ãų °ÍÀ¸·Î º»´Ù.

 

À̹ۿ¡µµ, fed-batch ȯ°æ¿¡¼­ ÀçÁ¶ÇÕ ´Ü¹éÁú »ý»ê ¼¼Æ÷ÁÖ¸¦ ÀÌ¿ëÇÑ transcriptome°ú DHFR-mediated gene amplification ½Ã½ºÅÛ¿¡¼­ÀÇ transcriptome ºÐ¼®¿¡ ´ëÇÑ µ¿Çâ°ú Ç×ü ¼­¿­À» ÃÖÀûÈ­ ½ÃÅ°±â À§ÇÑ ¸ñÀûÀ¸·Î µµÀÔµÈ ½Ã½ºÅÛ »ý¹°Çаú Ç×ü »ý»ê CHO ¼¼Æ÷ÁÖ¸¦ ½Ã½ºÅÛ »ý¹°ÇÐÀ» ÀÌ¿ëÇÏ¿© ºÐ¼®ÇÑ ³»¿ëÀÌ ¼Ò°³µÇ¾ú´Ù.


Âü°í¹®Çå

-Xun Xu, et al. 2011. The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line. Nature biotechnology. 29, 735-741(2011)
-Wurm FM. 2004. Production of recombinant protein therapeutics in cultivated mammalian cells. Nature biotechnology. 22(11):1393-1398
-DeFrancesco L. 2011. Move over ZFNs. Nat Biotechnol 29(8):681-4.
-Sander JD, Maeder ML, Reyon D, Voytas DF, Joung JK, Dobbs D. 2010. ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool. Nucleic Acids Res 38(Web Server issue):W462-8.




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